Isoforms of contractile proteins

Cytoplasmic contractile proteins

In reviewing work on cytoplasmic contractile proteins and the contributions made to this field by The Journal of Cell Biology, some perspective is gained by first pointing out that most cell biologists were not aware of the existence of these important cellular constituents more than 12 years ago. Times have changed, and today cytoplasmic contractile protein research is one of the busiest areas...

Contractile Proteins of Cultured Cells

An actin-like protein was isolated from the acetone powder of glycerinated chick embryo fibroblasts and purified to electrophoretic homogeneity as shown by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The purified protein has a molecular weight of 45,500, resembles rabbit actin in electrophoretic mobility and ultrastructure, undergoes reversible globular-fibrous transformations, a...

Contractile Proteins of Cultured Cells

An actin-like protein was isolated from the acetone powder of glycerinated chick embryo fibroblasts and purified to electrophoretic homogeneity as shown by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The purified protein has a molecular weight of 45,500, resembles rabbit actin in electrophoretic mobility and ultrastructure, undergoes reversible globular-fibrous transformations, a...

Regulation of contractile proteins by phosphorylation.

Tension generation by threads of contractile proteins

Threads of contractile proteins were formed via extrusion and their isometric tensions and isotonic contraction velocities were measured. We obtained reproducible data by using a new and sensitive tensiometer. The force-velocity curves of actomyosin threads were similar to those of muscle, with isometric tensions of the order of 10g/cm2 and maximum contraction velocites of the order of 10(-2) l...